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Image Search Results
Journal: Advanced Science
Article Title: Identification of PRDX5 as A Target for The Treatment of Castration‐Resistant Prostate Cancer
doi: 10.1002/advs.202304939
Figure Lengend Snippet: Suppression of CRPC tumor growth by PRDX5 inhibitors A) LNCaP epiDTP and enzDTP cell survivals were reduced by POL with IC 50 of 4.71 µM and 4.15 µM, and by STA with IC 50 of 4.08 µM and 3.82 µM, respectively. B) PRDX5 protein level was not changed by the treatment of POL, STA alone, or in combination with EPI, ENZ as measured by Western blotting. β‐ACTIN protein was used as the loading control. C) Cellular PRDX5 activity was measured by enzymatic assay in the presence of POL, STA, EPI, PRDX5 protein, or siPRDX5 alone or in combination. PRDX5 activity was inhibited by POL, STA, and siPRDX5, but increased by PRDX5 protein. D) Cell death rate (%) was measured by LDH release assay in the presence of POL, STA, EPI, PRDX5 protein, or siPRDX5 alone or in combination. Data are expressed as mean ± std of triplicates. One‐way ANOVA with the Turkey test was performed. p <0.05 was considered significant and indicated by different letters. E) PRDX5 inhibitor slowed down CRPC progression in mice. Weight and photograph of the prostate are shown from MYC T mice (6 per group) treated with enzalutamide (ENZ), abiraterone (ABI), polaprezinc (POL), stachyose (STA), or in combinations. AP: anterior prostate lobe, DL: dorsolateral prostate lobe, VP: ventral prostate lobe. Student's t ‐test was performed, ** and ***indicate p <0.01 and p <0.001, respectively. F) Representative images of prostate histopathology. Age and treatment are labeled.
Article Snippet: Enzalutamide (MCE, HY‐70002, NJ, US), EPI‐001 (Selleck, S7955, TX, US), Auranofin (MCE, HY‐B1123, NJ, US),
Techniques: Western Blot, Control, Activity Assay, Enzymatic Assay, Lactate Dehydrogenase Assay, Histopathology, Labeling